Purified Anti-Human CD47 Antibody[B6H12], Functional Grade (E-AB-F14130)
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For research use only.
| Alternate Names | MER, Protein MER, Leukocyte surface antigen CD, Antigenic surface determinant protein OA, CD47, IAP, MER6, OA3, Integrin-associated protein, Leukocyte surface antigen CD47, Antigenic surface determinant protein OA3, Integrin-associated protein (IAP), Protein MER6, Antigen identified by monoclonal 1D8, CD 47, CD47 antigen, CD47 antigen (Rh-related antigen, CD47 glycoprotein, CD47 molecule, Integrin Associated Protein, Integrin associated signal transducer, integrin-associated signal transducer), MER 6, OA 3, OTTHUMP00000041152, OTTHUMP00000041153, Rh related antigen, Surface antigen identified by monoclonal 1D8 |
| Clone | |
| Leadtime | Inquire |
| Verified Samples | Verified Samples in FCM: Jurkat |
| Dilution | FCM 2 μg/mL(0.5×106-1×106 cells);Neut Reported in the literature |
| Background | CD47 (also integrin-associated protein/IAP and OA3) is a variably glycosylated, 40‑60 kDa atypical member of the Ig-Superfamily. It is expressed on almost all cell types, including erythrocytes. CD47 binds to TSP-1 and SIRP alpha, and forms a membrane complex with CD36 and alpha v beta 3. Mature human CD47 is a 305 amino acid (aa), five-transmembrane glycoprotein. None (Azide-Free,Low Endotoxin) are perfectly suited to be used in culture or in vivo (for nonhuman studies) for functional assays blocking, neutralizing, activation or depletion where the presence of azide may damage cells or exogenous endotoxin may signal or activate cells. |
| Immunogen | Recombinant Human CD47 protein |
| Abbre | CD47 |
| Swissprot | |
| Host | Mouse |
| Reactivity | Human |
| Clonality | Monoclonal |
| Purification Method | >98%, Protein A/G purified |
| Isotype | Mouse IgG1, κ |
| Isotype Control | Purified Mouse IgG1, κ Isotype Control[MOPC-21], Functional Grade |
| Applications |
FCM;Neut
|
| Research Areas | Immunology;Cancer |
| Concentration |
≥ 1 mg/mL
|
| Conjugation | None (AF/LE) |
| Storage Buffer | Sterile PBS, pH 7.2. < 1.0 EU per mg of the antibody as determined by the LAL method. |
| Storage | Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles. This preparation contains no preservatives, thus it should be handled under aseptic conditions. |
| Expiration Date | 12 months |
| Shipping | Ice bag |
| Application References | Sydney R Gordon, et al. Nature. 2017 May 25;545(7655):495-499. |
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